Development and Application of High-quality Recombinant Viral Proteins

Scale-up productions of the viral protein faced many challenges, including issues revolving around protein aggregation, degradation, and yield instability. Thus, we need to optimize different cultures, transfection, purification conditions and key parameters to facilitate large-volume scale-up production.

Using an in-house developed efficient vector expression platform, different proprietary reagents, high-density cell culture technology, controlled culture conditions and the addition of anti-aggregation agents, with refined SEC-HPLC and buffer optimization, we were able to optimize the expression and scale-up production of viral proteins.